Anti-Poliovirus type 1 VP3/Capsid protein VP3 Polyclonal Antibody (VK644014)

Name: Anti-Poliovirus type 1 VP3/Capsid protein VP3 Polyclonal Antibody
Brand: abinScience
SKU: VK644014
Price: 168 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

VK644014

Reactivité des espèces

Poliovirus type 1 (PV1)

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Poliovirus type 1 VP3/Capsid protein VP3 (Gly342-Gln579).

Cible

Genome polyprotein, P1, Capsid protein VP3

Purification

Purified by antigen affinity column.

Numéro d'accès

P03300

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Genome polyprotein (VP3/Capsid protein VP3) is a ~246 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host epithelial cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

1. Hogle, JM. et al. (1985) Science (New York, N.Y.) 229, 1358-65. PMID: 2994218

2. Strauss, M. et al. (2013) Journal of virology 87, 3903-14. PMID: 23365424

3. Strauss, M. et al. (2015) Journal of virology 89, 4143-57. PMID: 25631086

4. Brandenburg, B. et al. (2007) PLoS biology 5, e183. PMID: 17622193

5. Coyne, CB. et al. (2007) The EMBO journal 26, 4016-28. PMID: 17717529

6. Bergelson, JM. (2008) Trends in microbiology 16, 44-7. PMID: 18191571

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Poliovirus type 1 VP3/Capsid protein VP3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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