Anti-S100A9 Polyclonal Antibody (HY562014)

Name: Anti-S100A9 Polyclonal Antibody
Brand: abinScience
SKU: HY562014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY562014

説明

Anti-S100A9 Polyclonal Antibody (HY562014) is a rabbit polyclonal antibody detecting S100A9 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

種反応性

Human

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human S100A9 (Thr2-Pro114).

ターゲット

Protein S100-A9, CAGB, MRP-14, S100 calcium-binding protein A9, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, p14, Calprotectin L1H subunit, S100A9, Calgranulin-B, CFAG, MRP14

精製

Purified by antigen affinity column.

アクセッション番号

P06702

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Protein S100-A9 (S100A9) is a ~13 kDa protein. S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Also participates in regulatory T-cell differentiation together with CD69.

1. Ryckman, C. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 170, 3233-42. PMID: 12626582

2. Vogl, T. et al. (2004) Blood 104, 4260-8. PMID: 15331440

3. Nakatani, Y. et al. (2005) Mediators of inflammation 2005, 280-92. PMID: 16258195

4. Champaiboon, C. et al. (2009) The Journal of biological chemistry 284, 7078-90. PMID: 19122197

5. Simard, JC. et al. (2010) Journal of leukocyte biology 87, 905-14. PMID: 20103766

6. Simard, JC. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 186, 3622-31. PMID: 21325622

7. Miyasaki, KT. et al. (1993) Journal of dental research 72, 517-23. PMID: 8423249

8. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-S100A9 polyclonal antibody at 1ug/mL on various samples.
Lane 1: THP-1 cell lysate
Lane 2: MCF-7 cell lysate