ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
15.4 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Protein S100-A9, CAGB, MRP-14, S100 calcium-binding protein A9, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, p14, Calprotectin L1H subunit, S100A9, Calgranulin-B, CFAG, MRP14
Background
Protein S100-A9 (S100A9) is a ~13 kDa protein. S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Also participates in regulatory T-cell differentiation together with CD69.
1. Ryckman, C. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 170, 3233-42. PMID: 12626582
2. Vogl, T. et al. (2004) Blood 104, 4260-8. PMID: 15331440
3. Nakatani, Y. et al. (2005) Mediators of inflammation 2005, 280-92. PMID: 16258195
4. Champaiboon, C. et al. (2009) The Journal of biological chemistry 284, 7078-90. PMID: 19122197
5. Simard, JC. et al. (2010) Journal of leukocyte biology 87, 905-14. PMID: 20103766
6. Simard, JC. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 186, 3622-31. PMID: 21325622
7. Miyasaki, KT. et al. (1993) Journal of dental research 72, 517-23. PMID: 8423249
8. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human S100A9 Protein, N-His
Bioactivity
Detected by Anti-Human S100A9 Antibody (6B4) (Cat No.: HY562013) in indirect ELISA.
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