Anti-Human S100A9 Recombinant Antibody (6B4) (HY562013)

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20ug
50ug
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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY562013

Description

Anti-Human S100A9 Antibody (6B4) [6B4] (HY562013) is a mouse monoclonal antibody detecting S100A9 in ELISA, FCM, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

ELISA, FCM, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

6B4

Clonality

Monoclonal

Target

Protein S100-A9, CAGB, MRP-14, S100 calcium-binding protein A9, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, p14, Calprotectin L1H subunit, S100A9, Calgranulin-B, CFAG, MRP14

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P06702

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Protein S100-A9 (S100A9) is a ~13 kDa protein. S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Also participates in regulatory T-cell differentiation together with CD69.

1. Ryckman, C. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 170, 3233-42. PMID: 12626582

2. Vogl, T. et al. (2004) Blood 104, 4260-8. PMID: 15331440

3. Nakatani, Y. et al. (2005) Mediators of inflammation 2005, 280-92. PMID: 16258195

4. Champaiboon, C. et al. (2009) The Journal of biological chemistry 284, 7078-90. PMID: 19122197

5. Simard, JC. et al. (2010) Journal of leukocyte biology 87, 905-14. PMID: 20103766

6. Simard, JC. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 186, 3622-31. PMID: 21325622

7. Miyasaki, KT. et al. (1993) Journal of dental research 72, 517-23. PMID: 8423249

8. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369

Note

For research use only.

Images

Bioactivity

Detects Recombinant Human S100A9 Protein, N-His (Cat No.: HY562012) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Anti-Human S100A9 Antibody (6B4).