Anti-WT1 Polyclonal Antibody (HB628014)

Name: Anti-WT1 Polyclonal Antibody
Brand: abinScience
SKU: HB628014
Price: 168 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HB628014

Description

Anti-WT1 Polyclonal Antibody (HB628014) is a rabbit polyclonal antibody detecting WT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Pig, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human WT1 (Thr317-Gln446).

Cible

WT1, Wilms tumor protein, WT33

Purification

Purified by antigen affinity column.

Numéro d'accès

P19544

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Wilms tumor protein (WT1) is a ~49 kDa protein. Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

1. Hamilton, TB. et al. (1995) Nucleic acids research 23, 277-84. PMID: 7862533

2. Stoll, R. et al. (2007) Journal of molecular biology 372, 1227-45. PMID: 17716689

3. Hashimoto, H. et al. (2014) Genes & development 28, 2304-13. PMID: 25258363

4. Smolen, GA. et al. (2004) Cancer research 64, 7846-51. PMID: 15520190

5. Markus, MA. et al. (2006) Experimental cell research 312, 3379-88. PMID: 16934801

6. Weiss, TC. et al. (2009) Biochemistry 48, 148-55. PMID: 19123921

Note

For research use only.

Images

Reférences

Fiche technique

Anti-WT1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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