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Anti-Human WT1 Recombinant Antibody (SAA1594) (HB628013)

Anti-Human WT1 Recombinant Antibody (SAA1594)
Anti-Human WT1 Recombinant Antibody (SAA1594)
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Overview
Catalog No.HB628013
Species reactivityHuman
ApplicationsELISA
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA1594
Clonality Monoclonal
Target WT1, Wilms tumor protein, WT33
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P19544
RRID Anti-Human WT1 Antibody (SAA1594) (abinScience Cat# HB628013, RRID:AB_3727355)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Wilms tumor protein (WT1) is a ~49 kDa protein. Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

1. Hamilton, TB. et al. (1995) Nucleic acids research 23, 277-84. PMID: 7862533
2. Stoll, R. et al. (2007) Journal of molecular biology 372, 1227-45. PMID: 17716689
3. Hashimoto, H. et al. (2014) Genes & development 28, 2304-13. PMID: 25258363
4. Smolen, GA. et al. (2004) Cancer research 64, 7846-51. PMID: 15520190
5. Markus, MA. et al. (2006) Experimental cell research 312, 3379-88. PMID: 16934801
6. Weiss, TC. et al. (2009) Biochemistry 48, 148-55. PMID: 19123921
Note For research use only.
Images
  • Anti-Human WT1 Recombinant Antibody (SAA1594)

    SDS-PAGE

    SDS-PAGE for Anti-Human WT1 Antibody (SAA1594)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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