Human WT1 Recombinant Protein (N-His) (HB628012)

Name: Human WT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB628012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB628012
Description	Recombinant Human WT1 Protein, N-His (HB628012) expressed in E. coli, spanning Thr317-Gln446. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P19544
Protein length	Thr317-Gln446
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	17.77 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	WT1, Wilms tumor protein, WT33
Background	Wilms tumor protein (WT1) is a ~49 kDa protein. Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. 1. Hamilton, TB. et al. (1995) Nucleic acids research 23, 277-84. PMID: 7862533 2. Stoll, R. et al. (2007) Journal of molecular biology 372, 1227-45. PMID: 17716689 3. Hashimoto, H. et al. (2014) Genes & development 28, 2304-13. PMID: 25258363 4. Smolen, GA. et al. (2004) Cancer research 64, 7846-51. PMID: 15520190 5. Markus, MA. et al. (2006) Experimental cell research 312, 3379-88. PMID: 16934801 6. Weiss, TC. et al. (2009) Biochemistry 48, 148-55. PMID: 19123921
Note	For research use only.