Anti-WT1 Polyclonal Antibody (HB628014)

Name: Anti-WT1 Polyclonal Antibody
Brand: abinScience
SKU: HB628014
Price: 168 USD
Availability: InStock

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Datasheet
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Overview

Catalog No.

HB628014

Description

Anti-WT1 Polyclonal Antibody (HB628014) is a rabbit polyclonal antibody detecting WT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Pig, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Alligator mississippiensis, Sminthopsis macroura, Pig, Xenopus laevis, Xenopus tropicalis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WT1 (Thr317-Gln446).

Target

WT1, Wilms tumor protein, WT33

Purification

Purified by antigen affinity column.

Accession

P19544

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Wilms tumor protein (WT1) is a ~49 kDa protein. Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

1. Hamilton, TB. et al. (1995) Nucleic acids research 23, 277-84. PMID: 7862533

2. Stoll, R. et al. (2007) Journal of molecular biology 372, 1227-45. PMID: 17716689

3. Hashimoto, H. et al. (2014) Genes & development 28, 2304-13. PMID: 25258363

4. Smolen, GA. et al. (2004) Cancer research 64, 7846-51. PMID: 15520190

5. Markus, MA. et al. (2006) Experimental cell research 312, 3379-88. PMID: 16934801

6. Weiss, TC. et al. (2009) Biochemistry 48, 148-55. PMID: 19123921

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-WT1 polyclonal antibody at 1ug/mL on recombinant WT1 (Catalog No. HB628012).

References

Datasheet

Anti-WT1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-WT1 Polyclonal Antibody (HB628014)

Overview

Images

References

Datasheet

SDS

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