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| カタログ番号 | YP051018 |
|---|---|
| 説明 |
cAMP ELISA KIT (YP051018) for research applications.
|
| サンプルタイプ | Plasma, Serum |
| 感度 | 1.56 pmol/mL |
| レンジ | 1.56-200 pmol/mL |
| アクセッション番号 | CAS: 60-92-4 |
| アプリケーション | ELISA |
| 検出方法 | Colorimetric |
| アッセイタイプ | Quantitative |
| 回収率 | 80-120% |
| 発送 | 2-8 °C |
| 安定性と保存 | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition. |
| 仕様 | cAMP |
| 背景 | Adenosine 3', 5'-cyclic monophosphate (cAMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cAMP ELISA Kit is based on the competition between HRP-labeled cAMP and free cAMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cAMP MAb) binding sites. Because the concentration of the HRP-labeled cAMP is held constant while the concentration of cAMP varies, the amount of HRP-labeled cAMP that is able to bind to the cAMP MAb will be inversely proportional to the concentration of cAMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50(50% B/B0) of approximately 12 pmol/mL and a detection limit of approximately 0.15 pmol/mL. |
| 注意事項 | For research use only. |
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