HEK293T Human CD142/F3/TF Stable Cell Line (HB058818)

カタログ番号	HB058818
説明	HEK293T Human CD142/F3/TF Stable Cell Line (HB058818) is a stable cell line expressing CD142/F3/TF. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
アクセッション番号	P13726
アプリケーション	FCM
宿主種	HEK293T
種	Human
ターゲット	CD142/F3/TF
特性	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
安定性と保存	Frozen in liquid nitrogen.
仕様	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景	Tissue factor (CD142/F3) is a ~33 kDa protein. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142 is the therapeutic target of tisotumab vedotin (Tivdak).

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD142 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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