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| カタログ番号 | HB111818 |
|---|---|
| 説明 |
HEK293T Human VIPR1/VPAC1 Stable Cell Line (HB111818) is a stable cell line expressing VIPR1/VPAC1. Engineered for consistent, high-level target expression in research applications.
Highlights
|
| アクセッション番号 | P32241 |
| アプリケーション | FCM |
| 宿主種 | HEK293T |
| 種 | Human |
| ターゲット | VIPR1/VPAC1 |
| 検出方法 | Colorimetric |
| サンプルタイプ | Plasma, Serum |
| アッセイタイプ | Quantitative |
| レンジ | 1.56 - 100 ng/mL |
| 感度 | 0.25 ng/mL |
| 回収率 | 80-120% |
| 特性 | Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL) |
| 発送 | 2-8 ℃ |
| 安定性と保存 | Frozen in liquid nitrogen. |
| 仕様 | 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium |
| 背景 | Vasoactive intestinal polypeptide receptor 1 (VIPR1/VPAC1) is a ~51 kDa protein. G protein-coupled receptor activated by the neuropeptides vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (ADCYAP1/PACAP). Binds VIP and both PACAP27 and PACAP38 bioactive peptides with the following order of ligand affinity VIP = PACAP27 > PACAP38. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway. 1. Xu, Y. et al. (2022) Nature communications 13, 2272. PMID: 35477937 2. Piper, SJ. et al. (2022) Nature communications 13, 7013. PMID: 36385145 3. Couvineau, A. et al. (1994) Biochemical and biophysical research communications 200, 769-76. PMID: 8179610 |
| 注意事項 | For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein. |
Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human VIPR1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
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