InVivoMAb Anti-Human IL-2 Antibody (Iv0020) (HX011010)

Name: InVivoMAb Anti-Human IL-2 Antibody (Iv0020)
Brand: abinScience
SKU: HX011010
Price: 370 USD
Availability: InStock

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Vista general

Número de catálogo	HX011010
Descripción	InVivoMAb Anti-Human IL2 (Iv0020) [Iv0020] (HX011010) is a mouse monoclonal antibody detecting IL2 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Reactividad de especies	Human
Aplicaciones	ELISA, FCM, WB, Neutralization
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG1
ID de clon	Iv0020
Aplicaciones probadas	WB, FCM
Objetivo	IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Productos asociados	pH 7.4 Dilution Buffer
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P60568
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nota	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Imágenes

Bioactivity

Detects IL2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IL2 antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL2 antibody monoclonal antibody (Catalog # HX011010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human IL2 (Iv0020).
Western blot

Western blot analysis was performed using anti-human IL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL2 (Catalog No: HX011012)
Lane 2: negative control
Flow cytometry

Flow-cytometry using PE anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.