Anti-Human IL2 Reference Antibody (MT204, RUO) (HX011046)

Name: Anti-Human IL2 Reference Antibody (MT204, RUO)
Brand: abinScience
SKU: HX011046
Price: 370 USD
Availability: InStock

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Overview

Catalog No.	HX011046
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG
Clone ID	MT204
Biological activity	MT204 (1-100 ug/mL, 4 days) dose-dependently inhibits proliferation and IFN-γ secretion in rhesus PBMCs stimulated by the mitogen concavalin A [1]. MT204 can block CD25-bound IL-2 on high-affinity IL-2 receptors with a k d of 0.58 nM [1] [2]. MT204 (5 h) dose dependently reduces the IL-2 inducible cell surface expression of the alpha subunit of IL-4 receptor (CD124) in CTLL-2 cells [2]. MT204 (5 days) inhibits IL-2-induced proliferation of NKL cells and T cells with IC 50 s of approximately 1.3 nM and 0.21 nM, respectively [2]. MT204 (15 min) potently antagonizes downstream signaling of IL-2 at sub-nanomolar concentrations, significantly reducing cellular pY-STAT3 levels stimulated by IL-2 in Primary human lymphocytes [2]. MT204 significantly inhibits IL-2 induced proliferation independently of CD25 expression levels in NKL cells, T cells and all NK cells with IC 50 s of 0.35-0.36 nM, 0.33 nM, and 0.1-0.13 nM, respectively [2]. Parmacokinetics Species Dose Route Note Plasma Concentration T 1/2 Rhesus monkey 2, 10, 50 or repeat 50 mg/kg i.v. 1 h infusion 1 mg/mL 326.4 h MT204 (50 mg/kg, i.v., day 0, day 5 and day 12 at a speed of 1.5 mL/kg/h over 1 h) significant delays in rejection of allogeneic skin graft in allogeneic skin graft model of rhesus monkey [1].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P60568
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	MT204
Background	Interleukin-2 (IL2) is a ~17 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. 1. Mingari, MC. et al. (1984) Nature 312, 641-3. PMID: 6438535 2. Wang, X. et al. (2005) Science (New York, N.Y.) 310, 1159-63. PMID: 16293754 3. Stauber, DJ. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 2788-93. PMID: 16477002 4. Miyazaki, T. et al. (1994) Science (New York, N.Y.) 266, 1045-7. PMID: 7973659 5. Kirken, RA. et al. (1995) Cytokine 7, 689-700. PMID: 8580378 6. Osinalde, N. et al. (2015) Proteomics 15, 520-31. PMID: 25142963 7. Holbrook, NJ. et al. (1984) Proceedings of the National Academy of Sciences of the United States of America 81, 1634-8. PMID: 6608729
References	1. Plater-Zyberk C, et al. The interleukin-2 antagonizing antibody MT204 delays allogeneic skin graft rejection in non-human primates and is well tolerated. Transpl Immunol. 2011 Sep; 25(2-3):133-40. [HX011046] 2. Volkland J, et al. A humanized monoclonal antibody against interleukin-2 that can inactivate the cytokine/receptor complex. Mol Immunol. 2007 Mar; 44(7):1743-53. [HX011046]
Note	For research use only. Not suitable for clinical or therapeutic use.