InVivoMAb Anti-Human IL-2 Antibody (Iv0020) (HX011010)

Name: InVivoMAb Anti-Human IL-2 Antibody (Iv0020)
Brand: abinScience
SKU: HX011010
Price: 370 USD
Availability: InStock

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概要

カタログ番号	HX011010
説明	InVivoMAb Anti-Human IL2 (Iv0020) [Iv0020] (HX011010) is a mouse monoclonal antibody detecting IL2 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
種反応性	Human
アプリケーション	ELISA, FCM, WB, Neutralization
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG1
クローンID	Iv0020
テスト済みアプリケーション	WB, FCM
ターゲット	IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
関連製品	pH 7.4 Dilution Buffer
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P60568
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

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Bioactivity

Detects IL2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IL2 antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL2 antibody monoclonal antibody (Catalog # HX011010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human IL2 (Iv0020).
Western blot

Western blot analysis was performed using anti-human IL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL2 (Catalog No: HX011012)
Lane 2: negative control
Flow cytometry

Flow-cytometry using PE anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.