InVivoMAb Anti-Human IL-2 Antibody (Iv0020) (HX011010)

Name: InVivoMAb Anti-Human IL-2 Antibody (Iv0020)
Brand: abinScience
SKU: HX011010
Price: 370 USD
Availability: InStock

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Спецификация:

100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер	HX011010
Описание	InVivoMAb Anti-Human IL2 (Iv0020) [Iv0020] (HX011010) is a mouse monoclonal antibody detecting IL2 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Реактивность видов	Human
Применения	ELISA, FCM, WB, Neutralization
Хозяинский вид	Mouse
Клоональность	Monoclonal
Изотип	IgG1
ID клона	Iv0020
Проверенные приложения	WB, FCM
Цель	IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Связанные продукты	pH 7.4 Dilution Buffer
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P60568
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Примечание	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Изображения

Bioactivity

Detects IL2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IL2 antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL2 antibody monoclonal antibody (Catalog # HX011010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human IL2 (Iv0020).
Western blot

Western blot analysis was performed using anti-human IL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL2 (Catalog No: HX011012)
Lane 2: negative control
Flow cytometry

Flow-cytometry using PE anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.