InVivoMAb Anti-Human IL-6 Antibody (Iv0022) (HY328010)

Name: InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
Brand: abinScience
SKU: HY328010
Price: 370 USD
Availability: InStock

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Precio(USD):

Especificación:

100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY328010

Descripción

InVivoMAb Anti-Human IL6 (Iv0022) [Iv0022] (HY328010) is a human monoclonal antibody detecting Hybridoma growth factor in ELISA, FCM, Neutralization. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Reactividad de especies

Human

Aplicaciones

ELISA, FCM, WB, Neutralization

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

Iv0022

Aplicaciones probadas

WB, FCM

Objetivo

B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, Interleukin-6

Nivel de endotoxinas

< 1 EU/mg

Pureza

>95% as determined by SDS-PAGE.

Productos asociados

pH 7.4 Dilution Buffer

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P05231

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Nota

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Imágenes

Bioactivity

SEC-HPLC detection for InVivoMAb Anti-Human IL6 (ABV0244).
Flow Cytometry

Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human IL6 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human IL6 (Iv0022).
Western blot

Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL6 (Catalog No: HY328021), 50ng
Lane 2: recombinant human IL6 (Catalog No: HY328021), 30ng
Lane 3: recombinant human IL6 (Catalog No: HY328021), 20ng
Lane 4: recombinant human IL6 (Catalog No: HY328021), 10ng