InVivoMAb Anti-Human IL-6 Antibody (Iv0022) (HY328010)

Name: InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
Brand: abinScience
SKU: HY328010
Price: 370 USD
Availability: InStock

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Prix(USD) :

Spécification :

100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY328010

Description

InVivoMAb Anti-Human IL6 (Iv0022) [Iv0022] (HY328010) is a human monoclonal antibody detecting Hybridoma growth factor in ELISA, FCM, Neutralization. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Reactivité des espèces

Human

Applications

ELISA, FCM, WB, Neutralization

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1, kappa

ID de clone

Iv0022

Applications testées

WB, FCM

Cible

B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, Interleukin-6

Niveau d'endotoxines

< 1 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Produits associés

pH 7.4 Dilution Buffer

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P05231

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Images

Bioactivity

SEC-HPLC detection for InVivoMAb Anti-Human IL6 (ABV0244).
Flow Cytometry

Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human IL6 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human IL6 (Iv0022).
Western blot

Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL6 (Catalog No: HY328021), 50ng
Lane 2: recombinant human IL6 (Catalog No: HY328021), 30ng
Lane 3: recombinant human IL6 (Catalog No: HY328021), 20ng
Lane 4: recombinant human IL6 (Catalog No: HY328021), 10ng