







| Каталожный номер | HF909010 |
|---|---|
| Описание |
InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066) [Iv0066] (HF909010) is a human monoclonal antibody detecting Beta-nerve growth factor in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
|
| Реактивность видов | Human |
| Применения | ELISA, WB, FCM, Neutralization |
| Хозяинский вид | Human |
| Клоональность | Monoclonal |
| Изотип | IgG2, kappa |
| ID клона | Iv0066 |
| Проверенные приложения | WB, FCM |
| Цель | Beta-NGF, Beta-nerve growth factor, NGF, NGFB |
| Уровень эндотоксинов | < 1 EU/mg |
| Чистота | >95% as determined by SDS-PAGE. |
| Связанные продукты | pH 7.4 Dilution Buffer |
| Очистка | Protein A/G purified from cell culture supernatant. |
| Номер доступа | P01138 |
| Форма | Liquid |
| Буфер хранения | 0.01M PBS pH 7.4. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Примечание | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG2 Recombinant Isotype Control Antibody (HyHEL-10) (HV988107) |

SEC-HPLC detection for InVivoMAb Anti-Human NGF/Beta-NGF (ABV0233).

Flow-cytometry using anti-human NGF antibody.NCI-H460 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NGF antibody monoclonal antibody (Catalog # HF909010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066).

Western blot analysis was performed using anti-NGF monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human NGF (Catalog No: HF909011)
Lane 2: negative control




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