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InVivoMAb Anti-Human TSG101 Antibody (Iv0254) (HV181020)

InVivoMAb Anti-Human TSG101 Antibody (Iv0254)
InVivoMAb Anti-Human TSG101 Antibody (Iv0254)
InVivoMAb Anti-Human TSG101 Antibody (Iv0254)
InVivoMAb Anti-Human TSG101 Antibody (Iv0254)
InVivoMAb Anti-Human TSG101 Antibody (Iv0254)
InVivoMAb Anti-Human TSG101 Antibody (Iv0254)
Prix(USD) :
Spécification :
  • 100ug
  • 1mg
Nombre :
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Aperçu
Numéro de catalogueHV181020
Description
InVivoMAb Anti-Human TSG101 Antibody (Iv0254) [Iv0254] (HV181020) is a human monoclonal antibody detecting Tumor susceptibility gene 101 protein in ELISA, Neutralization. Suitable for Human.
Reactivité des espècesHuman
ApplicationsELISA, FCM, Neutralization
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG
ID de cloneIv0254
Cible Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Produits associés pH 7.4 Dilution Buffer
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès Q99816
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Tumor susceptibility gene 101 protein (TSG101) is a ~43 kDa protein. Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation.

1. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413
2. Nabhan, JF. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 4146-51. PMID: 22315426
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images
  • InVivoMAb Anti-Human TSG101 Antibody (Iv0254)

    SDS-PAGE

    SDS-PAGE for InVivoMAb Anti-Human TSG101 Antibody (Iv0254)

  • InVivoMAb Anti-Human TSG101 Antibody (Iv0254)

    Flow cytometry

    Flow-cytometry using APC anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TSG101 monoclonal antibody (Catalog HV181020, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • InVivoMAb Anti-Human TSG101 Antibody (Iv0254)

    Flow cytometry

    Flow-cytometry using PE anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TSG101 monoclonal antibody (Catalog HV181020, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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