InVivoMAb Anti-Human TSG101 Antibody (Iv0253) (HV181010)

Name: InVivoMAb Anti-Human TSG101 Antibody (Iv0253)
Brand: abinScience
SKU: HV181010
Price: 370 USD
Availability: InStock

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Spec:

100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV181010

Description

InVivoMAb Anti-Human TSG101 Antibody (Iv0253) [Iv0253] (HV181010) is a human monoclonal antibody detecting Tumor susceptibility gene 101 protein in ELISA, Neutralization. Suitable for Human.

Species reactivity

Human

Applications

ELISA, WB, FCM, Neutralization

Host species

Human

Isotype

IgG

Clone ID

Iv0253

Clonality

Monoclonal

Target

Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Associated products

pH 7.4 Dilution Buffer

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q99816

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tumor susceptibility gene 101 protein (TSG101) is a ~43 kDa protein. Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation.

1. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

2. Nabhan, JF. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 4146-51. PMID: 22315426

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

Western blot

Western blot analysis was performed using anti-TSG101 monoclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: K562 cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Lncap cell lysate
Lane 6: 293T cell lysate
Lane 7: Mouse brain lysate
Lane 8: Rat brain lysate
Lane 9: Rat heart lysate
Flow cytometry

Flow-cytometry using APC anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.