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| カタログ番号 | HV181020 | ||||
|---|---|---|---|---|---|
| 説明 |
InVivoMAb Anti-Human TSG101 Antibody (Iv0254) [Iv0254] (HV181020) is a human monoclonal antibody detecting Tumor susceptibility gene 101 protein in ELISA, Neutralization. Suitable for Human.
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| 種反応性 | Human | ||||
| アプリケーション | ELISA, FCM, Neutralization | ||||
| 宿主種 | Human | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG | ||||
| クローンID | Iv0254 | ||||
| ターゲット | Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101 | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 関連製品 | pH 7.4 Dilution Buffer | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | Q99816 | ||||
| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||
| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 背景 | Tumor susceptibility gene 101 protein (TSG101) is a ~43 kDa protein. Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. 1. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413 2. Nabhan, JF. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 4146-51. PMID: 22315426 | ||||
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. | ||||
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SDS-PAGE for InVivoMAb Anti-Human TSG101 Antibody (Iv0254)
Flow-cytometry using APC anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TSG101 monoclonal antibody (Catalog HV181020, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TSG101 monoclonal antibody (Catalog HV181020, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
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