Human ADAMTS5 Recombinant Protein (N-His) (HV374012)

Name: Human ADAMTS5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV374012
Price: 211 USD
Availability: InStock

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50ug
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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HV374012
説明	Recombinant Human ADAMTS5 Protein, N-His (HV374012) expressed in E. coli, spanning Glu485-Pro622. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
発現システム	E. coli
アクセッション番号	Q9UNA0
タンパク質長	Glu485-Pro622
アプリケーション	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種	Homo sapiens (Human)
性質	Recombinant
エンドトキシンレベル	Please contact with the lab for this information.
純度	>90% as determined by SDS-PAGE.
予測分子量	17.44 kDa
形態	Lyophilized
保存バッファー	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
再構成	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
発送	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	ADAMTS11, ADAM-TS 11, A disintegrin and metalloproteinase with thrombospondin motifs 11, ADAMTS-11, ADMP2, ADAM-TS 5, ADAMTS-5, ADMP-2, ADAMTS5, Aggrecanase-2, ADAM-TS5, A disintegrin and metalloproteinase with thrombospondin motifs 5
背景	A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) is a ~101 kDa protein. Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration. 1. Nakada, M. et al. (2005) Acta neuropathologica 110, 239-46. PMID: 16133547 2. Longpré, JM. et al. (2009) The international journal of biochemistry & cell biology 41, 1116-26. PMID: 18992360
注意事項	For research use only.