Recombinant Human CD121a/IL1R1 Protein, C-Fc (HB791011) expressed in Mammalian Cells, spanning Met1-Thr332. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии
Mammalian cells
Номер доступа
P14778
Длина белка
Met1-Thr332
Применения
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
64.25 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
IL-1RT1, IL-1R-alpha, mIL-1R1, CD121a, IL-1R-1, sIL-1RI, IL1RT1, IL1R1, sIL-1R1, IL1R, CD121 antigen-like family member A, Interleukin-1 receptor type I, Interleukin-1 receptor alpha, mIL-1RI, IL1RA, IL-1RT-1, Interleukin-1 receptor type 1, p80
Фон
Interleukin-1 receptor type 1 (CD121a/IL1R1) is a ~65 kDa protein. Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.
1. Mosley, B. et al. (1987) The Journal of biological chemistry 262, 2941-4. PMID: 2950091
2. Wang, Y. et al. (2023) Immunity 56, 1485-1501.e7. PMID: 37315560
3. Tominaga, K. et al. (2000) International immunology 12, 151-60. PMID: 10653850
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human CD121a/IL1R1 Protein, C-Fc
Bioactivity
Detects Human CD121a/IL1R1 Antibody (27F2) in indirect ELISAs.
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