Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Interleukin-6 receptor subunit alpha (CD126/IL6R) is a ~51 kDa protein. Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system.
1. Larsen, JV. et al. (2017) Molecular and cellular biology 37. PMID: 28265003
2. Kang, S. et al. (2019) Immunity 50, 1007-1023. PMID: 30995492
3. Spencer, S. et al. (2019) The Journal of experimental medicine 216, 1986-1998. PMID: 31235509
Nota
For research use only.
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Human CD126/IL6R/IL-6RA Protein, C-His
Western blot
Detected by Anti-CD126/IL6R/IL-6RA monoclonal antibody (Catalog No: HY583016)
Western blot
Detected by Anti-CD126/IL6R/IL-6RA monoclonal antibody (Catalog No: HY583107)
Bioactivity
Immobilized HY328011 Human IL6 Recombinant Protein (C-His-Avi) at 2 μg/mL(100 μL/well) can bind HY583011 Human CD126/IL6R/IL-6RA Recombinant Protein (C-His), the EC 50 is 1.83 μg/mL.
Bioactivity
Immobilized HY328021 Human IL6 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HY583011 Human CD126/IL6R/IL-6RA Recombinant Protein (C-His), the EC 50 is 815 ng/mL.
Bioactivity
Immobilized HY328031 Human IL6 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HY583011 Human CD126/IL6R/IL-6RA Recombinant Protein (C-His), the EC 50 is 231 ng/mL.
Bioactivity
Immobilized HY328051 Human IL6 Recombinant Protein (C-Strep) at 2 μg/mL(100 μL/well) can bind HY583011 Human CD126/IL6R/IL-6RA Recombinant Protein (C-His), the EC 50 is 1.55 μg/mL.
中文
English
한국어
日本語
Español
Français
Русский
