Anti-Human CD126/IL6R/IL-6RA Antibody (SAA0048) (HY583107)

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Datasheet

Overview

Catalog No.	HY583107
Description	Anti-Human CD126/IL6R/IL-6RA Antibody (SAA0048) [SAA0048] (HY583107) is a mouse monoclonal antibody detecting IL-6RA in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	SAA0048
Clonality	Monoclonal
Target	sIL6R, Interleukin-6 receptor subunit alpha, CD126, IL-6R 1, IL-6R subunit alpha, Membrane glycoprotein 80, gp80, IL6R, IL-6RA, IL-6 receptor subunit alpha, IL-6R-alpha
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P08887
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Interleukin-6 receptor subunit alpha (CD126/IL6R) is a ~51 kDa protein. Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. 1. Larsen, JV. et al. (2017) Molecular and cellular biology 37. PMID: 28265003 2. Kang, S. et al. (2019) Immunity 50, 1007-1023. PMID: 30995492 3. Spencer, S. et al. (2019) The Journal of experimental medicine 216, 1986-1998. PMID: 31235509
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human CD126 antibody.CD126 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD126 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD126 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 monoclonal antibody (Catalog: HY583107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD126/IL6RA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD126/IL6RA (Catalog No: HY583011)
Lane 2: negative control