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| カタログ番号 | HW567011 |
|---|---|
| 説明 |
Recombinant Human CD151 Protein, N-Fc (HW567011) expressed in Mammalian Cells, spanning Ala113-Arg221. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | Mammalian cells |
| アクセッション番号 | P48509 |
| タンパク質長 | Ala113-Arg221 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 40.29 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CD151, PETA-3, Membrane glycoprotein SFA-1, GP27, Tspan-24, Platelet-endothelial tetraspan antigen 3, CD151 antigen, Tetraspanin-24, TSPAN24 |
| 背景 | CD151 antigen is a ~28 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Plays a role in various cellular and molecular mechanism through its association with both integrin and non-integrin proteins. These interactions facilitate critical cellular functions, including cell-to-cell communication, wound healing, platelet aggregation, trafficking, cell motility, and angiogenesis. Via interaction with JAM-A/F11R and integrin ITGA3:ITGB1, promotes the recruitment of signaling molecules such as RAC1, CDC42 and RhoGTPases to facilitate the polarization of epithelial cells and the reorganization of the actin cytoskeleton, which are critical steps in cell migration process. Regulates the glycosylation pattern of ITGA3:ITGB1 thereby modulating its activity. 1. Zheng, ZZ. et al. (2007) The international journal of biochemistry & cell biology 39, 340-8. PMID: 17045834 2. Rocha-Perugini, V. et al. (2014) European journal of immunology 44, 1967-75. PMID: 24723389 3. Te Molder, L. et al. (2019) Journal of cell science 132. PMID: 31488507 4. Hong, IK. et al. (2012) The Journal of biological chemistry 287, 32027-39. PMID: 22843693 5. Thölmann, S. et al. (2022) Cellular and molecular life sciences : CMLS 79, 88. PMID: 35067832 6. Baldwin, G. et al. (2008) The Journal of biological chemistry 283, 35445-54. PMID: 18852263 7. Karamatic Crew, V. et al. (2004) Blood 104, 2217-23. PMID: 15265795 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CD151 Protein, N-Fc
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中国武漢市深敦寺路666号C棟、武漢、430206
