Recombinant Human CD244 Protein, C-Fc (HV513011) expressed in Mammalian Cells, spanning Met1-Arg221.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression
Mammalian cells
Numéro d'accès
Q9BZW8
Longueur de protéine
Met1-Arg221
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>95% as determined by SDS-PAGE.
Masse moléculaire prédite
50.94 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
SLAM family member 4, SLAMF4, h2B4, Signaling lymphocytic activation molecule 4, Natural killer cell receptor 2B4, NKR2B4, NAIL, NK cell type I receptor protein 2B4, CD244, NK cell activation-inducing ligand, 2B4
Contexte
Natural killer cell receptor 2B4 (CD244) is a ~41 kDa protein. Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor. Activating function implicates association with SH2D1A and FYN.
1. Nakajima, H. et al. (1999) European journal of immunology 29, 1676-83. PMID: 10359122
2. Speiser, DE. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 6165-70. PMID: 11714776
3. Valiante, NM. et al. (1993) The Journal of experimental medicine 178, 1397-406. PMID: 8376943
4. Eissmann, P. et al. (2005) Blood 105, 4722-9. PMID: 15713798
5. Parolini, S. et al. (2000) The Journal of experimental medicine 192, 337-46. PMID: 10934222
6. Sivori, S. et al. (2000) European journal of immunology 30, 787-93. PMID: 10741393
7. Sivori, S. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 4526-31. PMID: 11917118
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD244 Protein, C-Fc
Bioactivity
Immobilized HV513011 Human CD244 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HY573021 Human CD48 Recombinant Protein (C-His), the EC 50 is 506 ng/mL.
Bioactivity
Immobilized HY573011 Human CD48 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HV513011 Human CD244 Recombinant Protein (C-Fc), the EC 50 is 10.9 μg/mL.
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