Human CD276/B7-H3 Recombinant Protein (C-His) (HV612011)

Name: Human CD276/B7-H3 Recombinant Protein (C-His)
Brand: abinScience
SKU: HV612011
Price: 298 USD
Availability: InStock

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50ug
100ug
1mg

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SDS

Vista general

Número de catálogo	HV612011
Descripción	Recombinant Human CD276 Protein, C-His (HV612011) expressed in Mammalian Cells, spanning Met1-Thr461. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	Mammalian cells
Número de acceso	Q5ZPR3
Longitud de proteína	Met1-Thr461
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Aplicaciones probadas	WB
Objetivo	4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	49.37 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule
Fondo	CD276 antigen (B7-H3) is a ~57 kDa protein. May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. CD276 is the therapeutic target of omburtamab (clinical).
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human CD276 Protein, C-His
Bioactivity

Detects Ifinatamab in indirect ELISAs.
Bioactivity

Detects Mirzotamab in indirect ELISAs.
Bioactivity

Detected by Research Grade Obrindatamab in indirect ELISA.
Bioactivity

Detected by Research Grade Obrindatamab (Cat No.: HV612046) in indirect ELISA.
Western blot

Detected by Anti-CD276 monoclonal antibody (Catalog No: HV612046)
Western blot

Detected by Anti-CD276 monoclonal antibody (Catalog No: HW310016)
Bioactivity

Detected by Research Grade Vobramitamab (Catalog No: HV612066) in indirect ELISA