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| カタログ番号 | HB030011 |
|---|---|
| 説明 |
Recombinant Human CD36 Protein, C-His (HB030011) expressed in Mammalian Cells, spanning Gln35-Asn439.
Highlights
|
| 発現システム | Mammalian cells |
| アクセッション番号 | P16671 |
| タンパク質長 | Gln35-Asn439 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 予測分子量 | 50.41 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CD36, FAT, GPIV, Fatty acid translocase, GP4, PAS IV, Leukocyte differentiation antigen CD36, Platelet collagen receptor, Platelet glycoprotein IV, Thrombospondin receptor, Platelet glycoprotein 4, GP3B, Glycoprotein IIIb, GPIIIB, PAS-4 |
| 背景 | Platelet glycoprotein 4 (CD36) is a ~53 kDa protein. Multifunctional glycoprotein that acts as a receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). 1. Smith, J. et al. (2008) The Journal of biological chemistry 283, 13578-85. PMID: 18353783 2. Mitchell, RW. et al. (2011) Journal of neurochemistry 117, 735-46. PMID: 21395585 3. Tran, TT. et al. (2011) The Journal of biological chemistry 286, 25201-10. PMID: 21610069 4. Hao, JW. et al. (2020) Nature communications 11, 4765. PMID: 32958780 5. Masuda, D. et al. (2009) Journal of lipid research 50, 999-1011. PMID: 18753675 6. Keller, KL. et al. (2012) Obesity (Silver Spring, Md.) 20, 1066-73. PMID: 22240721 7. Mrizak, I. et al. (2015) The British journal of nutrition 113, 1330-7. PMID: 25822988 8. Stewart, CR. et al. (2010) Nature immunology 11, 155-61. PMID: 20037584 9. Triantafilou, M. et al. (2006) The Journal of biological chemistry 281, 31002-11. PMID: 16880211 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CD36 Protein, C-His
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