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Human CD7 Recombinant Protein (C-His) (HY302011)

Human CD7 Recombinant Protein (C-His)
Human CD7 Recombinant Protein (C-His)
Human CD7 Recombinant Protein (C-His)
Human CD7 Recombinant Protein (C-His)
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Spécification :
  • 50ug
  • 100ug
  • 1mg
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  • Aperçu

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  • Reférences

  • Fiche technique

  • SDS

Aperçu
Numéro de catalogueHY302011
Description
Recombinant Human CD7 Protein, C-His (HY302011) expressed in Mammalian Cells, spanning Met1-Pro180.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionMammalian cells
Numéro d'accèsP09564
Longueur de protéineMet1-Pro180
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Masse moléculaire prédite 19.89 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsGP40, CD7, T-cell surface antigen Leu-9, T-cell leukemia antigen, TP41, T-cell antigen CD7
Contexte

T-cell antigen CD7 is a ~25 kDa protein. Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors. Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. In turn, mediates the production of cytokines such as IL-2. On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels.

1. Rabinowich, H. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 517-26. PMID: 7506726
2. Lyman, SD. et al. (2000) The Journal of biological chemistry 275, 3431-7. PMID: 10652336
3. Carrel, S. et al. (1991) European journal of immunology 21, 1195-200. PMID: 1709867
Note For research use only.
Images
  • Human CD7 Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human CD7 Protein, C-His

  • Human CD7 Recombinant Protein (C-His)

    Bioactivity

    Detects Grisnilimab in indirect ELISAs.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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