中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HV338012 |
|---|---|
| 説明 |
Recombinant Human CD77/A4GALT Protein, N-His (HV338012) expressed in E. coli, spanning Gly44-Leu353. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9NPC4 |
| タンパク質長 | Gly44-Leu353 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 37.7 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Gb3 synthase, UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase, CD77 synthase, Alpha-1, 4-N-acetylglucosaminyltransferase, Lactosylceramide 4-alpha-galactosyltransferase, Alpha4Gal-T1, Alpha-1, 4-galactosyltransferase, A4GALT1, A4GALT, Globotriaosylceramide synthase, P1/Pk synthase, A14GALT |
| 背景 | Lactosylceramide 4-alpha-galactosyltransferase (CD77/A4GALT) is a ~40 kDa protein. Glycosyltransferase involved in biosynthesis of P1, P(k) and the rare NOR antigens of P1PK histo-blood group system. Catalyzes the transfer of galactose from UDP-alpha-D-galactose in an alpha1,4 linkage to lactosylceramide/beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) to produce globotriaosylceramide/globoside Gb3Cer (d18:1(4E)), also known as P(k) antigen or CD77. Globoside Gb3Cer/P(k)/CD77 is a root structure in biosynthesis pathway of neutral glycosphingolipids of the globo-series. Broadly expressed in cell membranes of immune cells and other tissues, Gb3Cer/P(k)/CD77 antigen may participate in mechanisms that regulate cell-cell interactions and cell differentiation. In germinal center B cells, Gb3Cer/CD77 interacts with CD19 to drive centroblast-to-centrocyte transition, B cell receptor complex assembly and T follicular helper cell differentiation. 1. Steffensen, R. et al. (2000) The Journal of biological chemistry 275, 16723-9. PMID: 10747952 2. Kojima, Y. et al. (2000) The Journal of biological chemistry 275, 15152-6. PMID: 10748143 3. Suchanowska, A. et al. (2012) The Journal of biological chemistry 287, 38220-30. PMID: 22965229 4. Kaczmarek, R. et al. (2016) Biochemical and biophysical research communications 470, 168-174. PMID: 26773500 5. Jacob, F. et al. (2018) Cancer research 78, 2952-2965. PMID: 29572228 6. Szymczak-Kulus, K. et al. (2021) The Journal of biological chemistry 296, 100299. PMID: 33460651 |
| 注意事項 | For research use only. |
SDS-PAGE for Human CD77/A4GALT Recombinant Protein (N-His)
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
