Anti-CD77/A4GALT Polyclonal Antibody (HV338014)

Name: Anti-CD77/A4GALT Polyclonal Antibody
Brand: abinScience
SKU: HV338014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV338014

Description

Anti-CD77/A4GALT Polyclonal Antibody (HV338014) is a rabbit polyclonal antibody detecting A4GALT in ELISA, IHC, WB. Suitable for Human, Pan troglodytes, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Gorilla gorilla gorilla, Pan troglodytes, Pongo pygmaeus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD77/A4GALT (Gly44-Leu353).

Target

Gb3 synthase, UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase, CD77 synthase, Alpha-1, 4-N-acetylglucosaminyltransferase, Lactosylceramide 4-alpha-galactosyltransferase, Alpha4Gal-T1, Alpha-1, 4-galactosyltransferase, A4GALT1, A4GALT, Globotriaosylceramide synthase, P1/Pk synthase, A14GALT

Purification

Purified by antigen affinity column.

Accession

Q9NPC4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lactosylceramide 4-alpha-galactosyltransferase (CD77/A4GALT) is a ~40 kDa protein. Glycosyltransferase involved in biosynthesis of P1, P(k) and the rare NOR antigens of P1PK histo-blood group system. Catalyzes the transfer of galactose from UDP-alpha-D-galactose in an alpha1,4 linkage to lactosylceramide/beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) to produce globotriaosylceramide/globoside Gb3Cer (d18:1(4E)), also known as P(k) antigen or CD77. Globoside Gb3Cer/P(k)/CD77 is a root structure in biosynthesis pathway of neutral glycosphingolipids of the globo-series. Broadly expressed in cell membranes of immune cells and other tissues, Gb3Cer/P(k)/CD77 antigen may participate in mechanisms that regulate cell-cell interactions and cell differentiation. In germinal center B cells, Gb3Cer/CD77 interacts with CD19 to drive centroblast-to-centrocyte transition, B cell receptor complex assembly and T follicular helper cell differentiation.

1. Steffensen, R. et al. (2000) The Journal of biological chemistry 275, 16723-9. PMID: 10747952

2. Kojima, Y. et al. (2000) The Journal of biological chemistry 275, 15152-6. PMID: 10748143

3. Suchanowska, A. et al. (2012) The Journal of biological chemistry 287, 38220-30. PMID: 22965229

4. Kaczmarek, R. et al. (2016) Biochemical and biophysical research communications 470, 168-174. PMID: 26773500

5. Jacob, F. et al. (2018) Cancer research 78, 2952-2965. PMID: 29572228

6. Szymczak-Kulus, K. et al. (2021) The Journal of biological chemistry 296, 100299. PMID: 33460651

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD77/A4GALT polyclonal antibody at 1ug/mL on recombinant CD77/A4GALT (Catalog No. HV338012).

References

Datasheet

Anti-CD77/A4GALT Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CD77/A4GALT Polyclonal Antibody (HV338014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us