Recombinant Human CD9 Protein, C-His (HB984021) expressed in Mammalian Cells, spanning Ser112-Ile195. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión
Mammalian cells
Número de acceso
P21926
Longitud de proteína
Ser112-Ile195
Aplicaciones
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>90% as determined by SDS-PAGE.
Peso molecular predicho
12.58 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CD9 antigen is a ~25 kDa protein. Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption.
1. Higginbottom, A. et al. (2003) Biochemical and biophysical research communications 311, 208-14. PMID: 14575715
2. Nakazawa, Y. et al. (2008) Blood 112, 1730-9. PMID: 18541721
3. Ikeyama, S. et al. (1993) The Journal of experimental medicine 177, 1231-7. PMID: 8478605
4. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480
5. Masellis-Smith, A. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2768-77. PMID: 7511626
Nota
For research use only.
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Human CD9 Protein, C-His
Bioactivity
Detects Human CD9 Antibody (SAA1391) in indirect ELISAs.
Bioactivity
Detected by Research Grade Anti-Human CD9 (AT14-012) (Cat No.: HB984016) in indirect ELISA.
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