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| カタログ番号 | HB951022 |
|---|---|
| 説明 |
Recombinant Human ERCC3 Protein, N-His-SUMO (HB951022) expressed in E. coli, spanning Lys504-Glu725. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P19447 |
| タンパク質長 | Lys504-Glu725 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 38.16 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | BTF2 p89, Basic transcription factor 2 89 kDa subunit, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, EC:5.6.2.4, ERCC3, General transcription and DNA repair factor IIH helicase/translocase subunit XPB, TFIIH 89 kDa subunit, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH p89, TFIIH subunit XPB, XPB, XPBC, Xeroderma pigmentosum group B-complementing protein |
| 背景 | General transcription and DNA repair factor IIH helicase/translocase subunit XPB is a ~89 kDa protein. ATP-dependent 3'-5' DNA helicase/translocase. Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex. When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening; it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor. 1. Coin, F. et al. (2007) Molecular cell 26, 245-56. PMID: 17466626 2. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682 3. Aibara, S. et al. (2021) Nature 594, 124-128. PMID: 33902107 4. Schaeffer, L. et al. (1993) Science (New York, N.Y.) 260, 58-63. PMID: 8465201 5. Hwang, JR. et al. (1996) The Journal of biological chemistry 271, 15898-904. PMID: 8663148 6. Tirode, F. et al. (1999) Molecular cell 3, 87-95. PMID: 10024882 7. van Vuuren, AJ. et al. (1994) The EMBO journal 13, 1645-53. PMID: 8157004 10. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769 |
| 注意事項 | For research use only |
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