Anti-Human ERCC3 Polyclonal Antibody (HB951014)

Name: Anti-Human ERCC3 Polyclonal Antibody
Brand: abinScience
SKU: HB951014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB951014

Description

Anti-Human ERCC3 Polyclonal Antibody (HB951014) is a rabbit polyclonal antibody detecting ERCC3. Suitable for Human, Rat, and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ERCC3 (Lys504-Glu725).

Target

BTF2 p89, Basic transcription factor 2 89 kDa subunit, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, EC:5.6.2.4, ERCC3, General transcription and DNA repair factor IIH helicase/translocase subunit XPB, TFIIH 89 kDa subunit, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH p89, TFIIH subunit XPB, XPB, XPBC, Xeroderma pigmentosum group B-complementing protein

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P19447

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

General transcription and DNA repair factor IIH helicase/translocase subunit XPB (ERCC3) is a ~89 kDa protein. ATP-dependent 3'-5' DNA helicase/translocase. Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex. When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening; it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor.

1. Coin, F. et al. (2007) Molecular cell 26, 245-56. PMID: 17466626

2. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682

3. Aibara, S. et al. (2021) Nature 594, 124-128. PMID: 33902107

4. Schaeffer, L. et al. (1993) Science (New York, N.Y.) 260, 58-63. PMID: 8465201

5. Hwang, JR. et al. (1996) The Journal of biological chemistry 271, 15898-904. PMID: 8663148

6. Tirode, F. et al. (1999) Molecular cell 3, 87-95. PMID: 10024882

7. van Vuuren, AJ. et al. (1994) The EMBO journal 13, 1645-53. PMID: 8157004

10. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769

Note

For research use only

Images

References

Datasheet

Anti-Human ERCC3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ERCC3 Polyclonal Antibody (HB951014)

Overview

Images

References

Datasheet

SDS

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