ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
20.91 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
TFIIH basal transcription factor complex 89 kDa subunit, Xeroderma pigmentosum group B-complementing protein, TFIIH subunit XPB, Basic transcription factor 2 89 kDa subunit, ERCC3, DNA excision repair protein ERCC-3, TFIIH p89, TFIIH 89 kDa subunit, DNA repair protein complementing XP-B cells, General transcription and DNA repair factor IIH helicase subunit XPB, XPB, BTF2 p89, XPBC
Background
General transcription and DNA repair factor IIH helicase/translocase subunit XPB (ERCC3) is a ~89 kDa protein. ATP-dependent 3'-5' DNA helicase/translocase. Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex. When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening; it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor.
1. Coin, F. et al. (2007) Molecular cell 26, 245-56. PMID: 17466626
2. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
3. Aibara, S. et al. (2021) Nature 594, 124-128. PMID: 33902107
4. Schaeffer, L. et al. (1993) Science (New York, N.Y.) 260, 58-63. PMID: 8465201
5. Hwang, JR. et al. (1996) The Journal of biological chemistry 271, 15898-904. PMID: 8663148
6. Tirode, F. et al. (1999) Molecular cell 3, 87-95. PMID: 10024882
7. van Vuuren, AJ. et al. (1994) The EMBO journal 13, 1645-53. PMID: 8157004
10. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human ERCC3 Protein, N-His
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