Human LRP5 Recombinant Protein (N-His) (HF797012)

Name: Human LRP5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF797012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HF797012
Описание	Recombinant Human LRP5 Protein, N-His (HF797012) expressed in E. coli, spanning Cys1332-Leu1397. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	O75197
Длина белка	Cys1332-Leu1397
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	9.26 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Low-density lipoprotein receptor-related protein 5, LRP5, LRP-5, LRP7, LRP-7, Low-density lipoprotein receptor-related protein 7, LR3
Фон	Low-density lipoprotein receptor-related protein 5 (LRP5) is a ~179 kDa protein. Acts as a coreceptor with members of the frizzled family of seven-transmembrane spanning receptors to transduce signal by Wnt proteins. Activates the canonical Wnt signaling pathway that controls cell fate determination and self-renewal during embryonic development and adult tissue regeneration. In particular, may play an important role in the development of the posterior patterning of the epiblast during gastrulation. During bone development, regulates osteoblast proliferation and differentiation thus determining bone mass. Mechanistically, the formation of the signaling complex between Wnt ligand, frizzled receptor and LRP5 coreceptor promotes the recruitment of AXIN1 to LRP5, stabilizing beta-catenin/CTNNB1 and activating TCF/LEF-mediated transcriptional programs. 1. Mao, J. et al. (2001) Molecular cell 7, 801-9. PMID: 11336703 2. Semënov, MV. et al. (2001) Current biology : CB 11, 951-61. PMID: 11448771 3. Gong, Y. et al. (2001) Cell 107, 513-23. PMID: 11719191 4. Li, X. et al. (2005) The Journal of biological chemistry 280, 19883-7. PMID: 15778503 5. Semënov, M. et al. (2005) The Journal of biological chemistry 280, 26770-5. PMID: 15908424 6. Ai, M. et al. (2005) American journal of human genetics 77, 741-53. PMID: 16252235
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human LRP5 Protein, N-His

Ссылки

Даташит

Human LRP5 Recombinant Protein (N-His).pdf

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Formula

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Stock Solution

C₁ (Stock Conc.)

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C₂ (Working Conc.)

V₂ (Working Vol.)

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