ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>90% as determined by SDS-PAGE.
Peso molecular predicho
15.71 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos
Amphiregulin-associated protein, Midgestation and kidney protein, NEGF2, MDK, ARAP, MK1, Neurite outgrowth-promoting factor 2, MK, Midkine, Neurite outgrowth-promoting protein
Fondo
Midkine (MDK) is a ~15 kDa protein. Secreted protein that functions as a cytokine and growth factor and mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptors via their chondroitin sulfate (CS) groups. Thereby regulates many processes like inflammatory response, cell proliferation, cell adhesion, cell growth, cell survival, tissue regeneration, cell differentiation and cell migration. Participates in inflammatory processes by exerting two different activities. Firstly, mediates neutrophils and macrophages recruitment to the sites of inflammation both by direct action by cooperating namely with ITGB2 via LRP1 and by inducing chemokine expression.
1. Maeda, N. et al. (1999) The Journal of biological chemistry 274, 12474-9. PMID: 10212223
2. Muramatsu, H. et al. (2000) Biochemical and biophysical research communications 270, 936-41. PMID: 10772929
3. Kurosawa, N. et al. (2001) Glycoconjugate journal 18, 499-507. PMID: 12084985
4. Stoica, GE. et al. (2002) The Journal of biological chemistry 277, 35990-8. PMID: 12122009
5. Sakaguchi, N. et al. (2003) Neuroscience research 45, 219-24. PMID: 12573468
6. Muramatsu, H. et al. (2004) Journal of cell science 117, 5405-15. PMID: 15466886
7. Huang, Y. et al. (2008) Cell cycle (Georgetown, Tex.) 7, 1613-22. PMID: 18469519
中文
English
한국어
日本語
Español
Français
Русский
