

| Каталожный номер | HP580032 |
|---|---|
| Описание |
Recombinant Human SGMS1 Protein, N-GST & C-His (HP580032) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | Q86VZ5 |
| Длина белка | Lys2-Ala81 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Homo sapiens (Human) |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | EC:2.7.8.27, MOB, Medulla oblongata-derived protein, Phosphatidylcholine:ceramide cholinephosphotransferase 1, Protein Mob, SGMS1, SMS1, Sphingomyelin synthase 1, TMEM23, Transmembrane protein 23 |
| Фон | Phosphatidylcholine:ceramide cholinephosphotransferase 1 is a ~48 kDa protein. Major sphingomyelin synthase at the Golgi apparatus. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction depends on the levels of CER and DAG in Golgi membranes. Converts the newly synthesized CER, that is transported from the endoplasmic reticulum to the trans-Golgi by the Cer transport protein (CERT), to SM. Can form a heteromeric complex with glucosylceramide synthase (GCS) increasing SMS activity and reducing glucosylceramide synthesis, a critical mechanism that controls the metabolic fate of CER in the Golgi. 1. Huitema, K. et al. (2004) The EMBO journal 23, 33-44. PMID: 14685263 2. Tafesse, FG. et al. (2007) The Journal of biological chemistry 282, 17537-47. PMID: 17449912 3. Yamaoka, S. et al. (2004) The Journal of biological chemistry 279, 18688-93. PMID: 14976195 4. Ding, T. et al. (2008) Journal of lipid research 49, 376-85. PMID: 17982138 5. Ternes, P. et al. (2009) Journal of lipid research 50, 2270-7. PMID: 19454763 6. Hayashi, Y. et al. (2018) The Journal of biological chemistry 293, 17505-17522. PMID: 30242129 |
| Примечание | For research use only |



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