Human SGMS1 Recombinant Protein (N-His) (HP580022)

Name: Human SGMS1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP580022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP580022
Description	Recombinant Human SGMS1 Protein, N-His (HP580022) expressed in E. coli, spanning Met1-Arg125. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q86VZ5
Protein length	Met1-Arg125
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.53 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:2.7.8.27, MOB, Medulla oblongata-derived protein, Phosphatidylcholine:ceramide cholinephosphotransferase 1, Protein Mob, SGMS1, SMS1, Sphingomyelin synthase 1, TMEM23, Transmembrane protein 23
Background	Phosphatidylcholine:ceramide cholinephosphotransferase 1 is a ~48 kDa protein. Major sphingomyelin synthase at the Golgi apparatus. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction depends on the levels of CER and DAG in Golgi membranes. Converts the newly synthesized CER, that is transported from the endoplasmic reticulum to the trans-Golgi by the Cer transport protein (CERT), to SM. Can form a heteromeric complex with glucosylceramide synthase (GCS) increasing SMS activity and reducing glucosylceramide synthesis, a critical mechanism that controls the metabolic fate of CER in the Golgi. 1. Huitema, K. et al. (2004) The EMBO journal 23, 33-44. PMID: 14685263 2. Tafesse, FG. et al. (2007) The Journal of biological chemistry 282, 17537-47. PMID: 17449912 3. Yamaoka, S. et al. (2004) The Journal of biological chemistry 279, 18688-93. PMID: 14976195 4. Ding, T. et al. (2008) Journal of lipid research 49, 376-85. PMID: 17982138 5. Ternes, P. et al. (2009) Journal of lipid research 50, 2270-7. PMID: 19454763 6. Hayashi, Y. et al. (2018) The Journal of biological chemistry 293, 17505-17522. PMID: 30242129
Note	For research use only

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Human SGMS1 Recombinant Protein (N-His).pdf

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Human SGMS1 Recombinant Protein (N-His) (HP580022)

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