Anti-Human SGMS1 Polyclonal Antibody (HP580014)

Name: Anti-Human SGMS1 Polyclonal Antibody
Brand: abinScience
SKU: HP580014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP580014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SGMS1 (Lys2-Ala81).

Target

Sphingomyelin synthase 1, Medulla oblongata-derived protein, Protein Mob, Transmembrane protein 23, Phosphatidylcholine:ceramide cholinephosphotransferase 1, TMEM23, MOB, SMS1, SGMS1

Purification

Purified by antigen affinity column.

Accession

Q86VZ5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phosphatidylcholine:ceramide cholinephosphotransferase 1 (SGMS1) is a ~48 kDa protein. Major sphingomyelin synthase at the Golgi apparatus. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction depends on the levels of CER and DAG in Golgi membranes. Converts the newly synthesized CER, that is transported from the endoplasmic reticulum to the trans-Golgi by the Cer transport protein (CERT), to SM. Can form a heteromeric complex with glucosylceramide synthase (GCS) increasing SMS activity and reducing glucosylceramide synthesis, a critical mechanism that controls the metabolic fate of CER in the Golgi.

1. Huitema, K. et al. (2004) The EMBO journal 23, 33-44. PMID: 14685263

2. Tafesse, FG. et al. (2007) The Journal of biological chemistry 282, 17537-47. PMID: 17449912

3. Yamaoka, S. et al. (2004) The Journal of biological chemistry 279, 18688-93. PMID: 14976195

4. Ding, T. et al. (2008) Journal of lipid research 49, 376-85. PMID: 17982138

5. Ternes, P. et al. (2009) Journal of lipid research 50, 2270-7. PMID: 19454763

6. Hayashi, Y. et al. (2018) The Journal of biological chemistry 293, 17505-17522. PMID: 30242129

Note

For research use only.

Images

References

Datasheet

Anti-Human SGMS1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human SGMS1 Polyclonal Antibody (HP580014)

Overview

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Datasheet

SDS

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