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| カタログ番号 | HC467012 |
|---|---|
| 説明 |
Recombinant Human TRIM32 Protein, N-His-SUMO & C-Strep (HC467012) expressed in E. coli, spanning Ser7-Arg101. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q13049 |
| タンパク質長 | Ser7-Arg101 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 24.20 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | E3 ubiquitin-protein ligase TRIM32, 2.3.2.27, 72 kDa Tat-interacting protein, RING-type E3 ubiquitin transferase TRIM32, Tripartite motif-containing protein 32, Zinc finger protein HT2A, TRIM32, HT2A |
| 背景 | E3 ubiquitin-protein ligase TRIM32 is a ~71 kDa protein. E3 ubiquitin ligase that plays a role in various biological processes including neural stem cell differentiation, innate immunity, inflammatory resonse and autophagy. Plays a role in virus-triggered induction of IFN-beta and TNF by mediating the ubiquitination of STING1. Mechanistically, targets STING1 for 'Lys-63'-linked ubiquitination which promotes the interaction of STING1 with TBK1. Regulates bacterial clearance and promotes autophagy in Mycobacterium tuberculosis-infected macrophages. Negatively regulates TLR3/4-mediated innate immune and inflammatory response by triggering the autophagic degradation of TICAM1 in an E3 activity-independent manner. 1. Locke, M. et al. (2009) Human molecular genetics 18, 2344-58. PMID: 19349376 2. Di Rienzo, M. et al. (2019) Science advances 5, eaau8857. PMID: 31123703 3. Zhang, J. et al. (2012) The Journal of biological chemistry 287, 28646-55. PMID: 22745133 4. Romagnoli, A. et al. (2023) Cell death & disease 14, 505. PMID: 37543647 5. Yang, Q. et al. (2017) PLoS pathogens 13, e1006600. PMID: 28898289 6. Prajapati, P. et al. (2020) Cellular signalling 76, 109777. PMID: 32918979 7. Ryu, YS. et al. (2011) The Journal of biological chemistry 286, 25729-38. PMID: 21628460 8. Zhang, Q. et al. (2012) The Journal of biological chemistry 287, 20625-35. PMID: 22500027 10. Liu, J. et al. (2023) Cell reports 42, 113402. PMID: 37943659 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human TRIM32 Protein, N-His-SUMO & C-Strep
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