Anti-Human TRIM32 Polyclonal Antibody (HC467014)

Name: Anti-Human TRIM32 Polyclonal Antibody
Brand: abinScience
SKU: HC467014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HC467014

Description

Anti-Human TRIM32 Polyclonal Antibody (HC467014) is a rabbit polyclonal antibody detecting TRIM32 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TRIM32 (Ser7-Arg101).

Target

E3 ubiquitin-protein ligase TRIM32, 2.3.2.27, 72 kDa Tat-interacting protein, RING-type E3 ubiquitin transferase TRIM32, Tripartite motif-containing protein 32, Zinc finger protein HT2A, TRIM32, HT2A

Purification

Purified by antigen affinity column.

Accession

Q13049

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase TRIM32 is a ~71 kDa protein. E3 ubiquitin ligase that plays a role in various biological processes including neural stem cell differentiation, innate immunity, inflammatory resonse and autophagy. Plays a role in virus-triggered induction of IFN-beta and TNF by mediating the ubiquitination of STING1. Mechanistically, targets STING1 for 'Lys-63'-linked ubiquitination which promotes the interaction of STING1 with TBK1. Regulates bacterial clearance and promotes autophagy in Mycobacterium tuberculosis-infected macrophages. Negatively regulates TLR3/4-mediated innate immune and inflammatory response by triggering the autophagic degradation of TICAM1 in an E3 activity-independent manner.

1. Locke, M. et al. (2009) Human molecular genetics 18, 2344-58. PMID: 19349376

2. Di Rienzo, M. et al. (2019) Science advances 5, eaau8857. PMID: 31123703

3. Zhang, J. et al. (2012) The Journal of biological chemistry 287, 28646-55. PMID: 22745133

4. Romagnoli, A. et al. (2023) Cell death & disease 14, 505. PMID: 37543647

5. Yang, Q. et al. (2017) PLoS pathogens 13, e1006600. PMID: 28898289

6. Prajapati, P. et al. (2020) Cellular signalling 76, 109777. PMID: 32918979

7. Ryu, YS. et al. (2011) The Journal of biological chemistry 286, 25729-38. PMID: 21628460

8. Zhang, Q. et al. (2012) The Journal of biological chemistry 287, 20625-35. PMID: 22500027

10. Liu, J. et al. (2023) Cell reports 42, 113402. PMID: 37943659

Note

For research use only.

Images

References

Datasheet

Anti-Human TRIM32 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human TRIM32 Polyclonal Antibody (HC467014)

Overview

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References

Datasheet

SDS

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