Human WDR5 Recombinant Protein (N-His) (HX013012)

Name: Human WDR5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX013012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HX013012
Descripción	Recombinant Human WDR5 Protein, N-His (HX013012) expressed in E. coli, spanning Met1-Lys120. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P61964
Longitud de proteína	Met1-Lys120
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	14.99 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	WDR5, BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, BIG3
Fondo	WD repeat-containing protein 5 (WDR5) is a ~36 kDa protein. Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. 1. Han, Z. et al. (2006) Molecular cell 22, 137-44. PMID: 16600877 2. Couture, JF. et al. (2006) Nature structural & molecular biology 13, 698-703. PMID: 16829960 3. Guelman, S. et al. (2009) Molecular and cellular biology 29, 1176-88. PMID: 19103755 4. Garapaty, S. et al. (2009) The Journal of biological chemistry 284, 7542-52. PMID: 19131338 5. Patel, A. et al. (2009) The Journal of biological chemistry 284, 24242-56. PMID: 19556245 6. Cai, Y. et al. (2010) The Journal of biological chemistry 285, 4268-72. PMID: 20018852 7. Song, JJ. et al. (2008) The Journal of biological chemistry 283, 35258-64. PMID: 18840606 8. Avdic, V. et al. (2011) Structure (London, England : 1993) 19, 101-8. PMID: 21220120 9. Zhang, P. et al. (2012) Nucleic acids research 40, 4237-46. PMID: 22266653
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human WDR5 Protein, N-His

Referencias

Datasheet

Human WDR5 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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