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Influenza A virus PA/Polymerase Acidic Recombinant Protein (N-His-SUMO) (VK560012)

Influenza A virus PA/Polymerase Acidic Recombinant Protein (N-His-SUMO)
Influenza A virus PA/Polymerase Acidic Recombinant Protein (N-His-SUMO)
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  • 概要

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  • データシート

  • SDS

概要
カタログ番号VK560012
説明
Recombinant Influenza A virus PA/Polymerase Acidic Protein, N-His-SUMO (VK560012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
発現システムE. coli
アクセッション番号P03433
タンパク質長Glu2-Gly209
アプリケーションELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種Influenza A virus (H1N1)
性質 Recombinant
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名EC:3.1.-.-, PA, Polymerase acidic protein, RNA-directed RNA polymerase subunit P2
背景

Polymerase acidic protein is a ~82 kDa protein. Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.

注意事項 For research use only
イメージ
  • Influenza A virus PA/Polymerase Acidic Recombinant Protein (N-His-SUMO)

    SDS-PAGE

    SDS-PAGE for Recombinant Influenza A virus (H1N1) Polymerase PA Protein, N-His-SUMO

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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