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Anti-Influenza A virus PA/Polymerase Acidic Protein Polyclonal Antibody (VK560014)

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Overview
Catalog No.VK560014
Description
Anti-Influenza A virus PA/Polymerase Acidic Protein Polyclonal Antibody (VK560014) is a rabbit polyclonal antibody detecting Polymerase acidic protein. Suitable for Influenza A virus.
Species reactivityInfluenza A virus
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Influenza A virus PA/Polymerase Acidic Protein (Glu2-Gly209).
Target EC:3.1.-.-, PA, Polymerase acidic protein, RNA-directed RNA polymerase subunit P2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P03433
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Polymerase acidic protein (PA) is a ~82 kDa protein. Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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