Influenza A virus (H1N1) Polymerase PA Recombinant Protein (N-His-SUMO) (VK560022)

Name: Influenza A virus (H1N1) Polymerase PA Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: VK560022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK560022
Description	Recombinant Influenza A virus (H1N1) Polymerase PA Protein, N-His-SUMO (VK560022) expressed in E. coli, spanning Glu2-Gly209. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P03433
Protein length	Glu2-Gly209
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Influenza A virus (H1N1)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.86 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:3.1.-.-, PA, Polymerase acidic protein, RNA-directed RNA polymerase subunit P2
Background	Polymerase acidic protein is a ~82 kDa protein. Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.
Note	For research use only

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Influenza A virus (H1N1) Polymerase PA Recombinant Protein (N-His-SUMO).pdf

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Influenza A virus (H1N1) Polymerase PA Recombinant Protein (N-His-SUMO) (VK560022)

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