中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | VK654011 |
|---|---|
| 説明 |
Recombinant MeV H/Hemagglutinin glycoprotein Protein, C-His (VK654011) expressed in Mammalian Cells, spanning Arg59-Arg617. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | Mammalian cells |
| アクセッション番号 | P35971 |
| タンパク質長 | Arg59-Arg617 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Measles virus (MeV) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 65.61 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Hemagglutinin glycoprotein, H |
| 背景 | Hemagglutinin glycoprotein is a ~69 kDa protein. Attaches the virus to the human SLAMF1/CD150 receptor for entry into host dendritic cells, macrophages, activated memory T cells and naive or memory B cells, thereby explaining the long immunosuppression that follows infection. In the respiratory airways, binds to the NECTIN4 receptor for entry into the host cell. Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. The vaccine and laboratory-adapted strains use host CD46 as an alternate receptor. The high degree of interaction between H and CD46 results in down-regulation of the latter from the surface of infected cells, rendering them more sensitive to c3b-mediated complement lysis. 1. Erlenhöfer, C. et al. (2002) The Journal of general virology 83, 1431-1436. PMID: 12029158 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant MeV H/Hemagglutinin glycoprotein Protein, C-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
