MeV Hemagglutinin glycoprotein Recombinant Protein (N-His) (VK654012)

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Overview

Catalog No.	VK654012
Description	Recombinant MeV Hemagglutinin glycoprotein, N-His (VK654012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	P35971
Protein length	Arg59-Arg617
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Measles virus (strain Edmonston-AIK-C vaccine) (MeV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	H, Hemagglutinin glycoprotein
Background	Hemagglutinin glycoprotein is a ~69 kDa protein. Attaches the virus to the human SLAMF1/CD150 receptor for entry into host dendritic cells, macrophages, activated memory T cells and naive or memory B cells, thereby explaining the long immunosuppression that follows infection. In the respiratory airways, binds to the NECTIN4 receptor for entry into the host cell. Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. The vaccine and laboratory-adapted strains use host CD46 as an alternate receptor. The high degree of interaction between H and CD46 results in down-regulation of the latter from the surface of infected cells, rendering them more sensitive to c3b-mediated complement lysis. 1. Erlenhöfer, C. et al. (2002) The Journal of general virology 83, 1431-1436. PMID: 12029158
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant MeV H/Hemagglutinin glycoprotein Protein, N-His

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Datasheet

MeV Hemagglutinin glycoprotein Recombinant Protein (N-His).pdf

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MeV Hemagglutinin glycoprotein Recombinant Protein (N-His) (VK654012)

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Datasheet

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