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MeV H/Hemagglutinin glycoprotein Recombinant Protein (C-His) (VK654011)

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MeV H/Hemagglutinin glycoprotein Recombinant Protein (C-His)

MeV H/Hemagglutinin glycoprotein Recombinant Protein (C-His)

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Datasheet
SDS

Overview

Catalog No.	VK654011
Description	Recombinant MeV H/Hemagglutinin glycoprotein Protein, C-His (VK654011) expressed in Mammalian Cells, spanning Arg59-Arg617. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P35971
Protein length	Arg59-Arg617
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Measles virus (strain Edmonston-AIK-C vaccine) (MeV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Hemagglutinin glycoprotein, H
Background	Hemagglutinin glycoprotein is a ~69 kDa protein. Attaches the virus to the human SLAMF1/CD150 receptor for entry into host dendritic cells, macrophages, activated memory T cells and naive or memory B cells, thereby explaining the long immunosuppression that follows infection. In the respiratory airways, binds to the NECTIN4 receptor for entry into the host cell. Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. The vaccine and laboratory-adapted strains use host CD46 as an alternate receptor. The high degree of interaction between H and CD46 results in down-regulation of the latter from the surface of infected cells, rendering them more sensitive to c3b-mediated complement lysis. 1. Erlenhöfer, C. et al. (2002) The Journal of general virology 83, 1431-1436. PMID: 12029158
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant MeV H/Hemagglutinin glycoprotein Protein, C-His

References

VK654011-SDS-PAGE-1.jpg

Datasheet

MeV H/Hemagglutinin glycoprotein Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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MeV H/Hemagglutinin glycoprotein Recombinant Protein (C-His)